【目录】
综述 —— 3
・ “何谓外泌体?” 金泽大学 纳米生命科学研究所 华山力成 教授············································································ 3
PS亲和法 —— 5
・ 前言························································································································································································· 5
・ 磷脂酰丝氨酸和Tim4新型外泌体提取法························································································································· 5
・ 外泌体分离和纯化方法的比较··········································································································································· 5
・ PS亲和法的性能数据··························································································································································· 6
・ 【专栏】不能仅凭蛋白总量计算外泌体回收量的原因·································································································· 9
・ 金泽大学 纳米生命科学研究所 华山教授问答集《关于PS亲和法的优点与常见问题》········································· 9
外泌体分离和纯化 —— 11
・ MagCapture™ 外泌体提取试剂盒PS Ver.2 ·················································································································· 11
miRNA提取和纯化 —— 13
・ 纯化细胞外囊泡(EV)用miRNA提取试剂盒················································································································· 13
外泌体检测和定量 —— 14
・ 使用PS亲和法开发ELISA试剂盒·········································································································································· 14
・ 外泌体ELISA试剂盒选择表··················································································································································· 14
・ PS Capture™ 外泌体ELISA试剂盒(抗小鼠IgG POD)······························································································· 15
・ PS Capture™ 外泌体ELISA试剂盒(链霉亲和素HRP)································································································ 16
・ CD9/63/81-Capture人外泌体ELISA试剂盒(链霉亲和素HRP)··············································································· 18
・ PS Capture™ 外泌体流式试剂盒········································································································································ 20
・ 抗外泌体标记物抗体(CD9/CD63/CD81)····················································································································· 22
外泌体的吸附抑制和冷冻保存剂 —— 24
・ EV-Save™ 细胞外囊泡保存稳定剂······································································································································ 24
・ EV-Save™ 细胞外囊泡保存稳定剂(动物体内实验用)································································································· 25
纯化外泌体—— 26
・ COLO201细胞来源纯化物外泌体········································································································································ 26
培养基和培养器具——27
・MSCulture™ High Growth基础培养基 / MSCulture™ High Growth添加剂··························································· 27
・EV-Up™ 间充质干细胞外泌体生产用基础培养基,AF / EV-Up™ 间充质干细胞外泌体生产用添加剂,AF········ 28
・UniWells™ 水平连接细胞共培养板······································································································································· 30
常见问题 —— 32
相关产品:
2. PS Capture™ 外泌体ELISA试剂盒(抗小鼠IgG POD)
6. PS Capture™ 外泌体ELISA试剂盒(链霉亲和素HRP)
11.水平共培养板连接器Connector for horizontal plate
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