生产系统

转基因 CHO-S 细胞

转基因蚕生产系统

提纯材料

CHO-S 细胞培养上清

蚕蛹蛋白

产品等级

实验研究用* *有临床用级别

实验研究用

导入基因

人层粘连蛋白 511-E8 片段

纯度

95% 以上

浓度

0.5 mg/mL

解离常数

10 nM 以下

使用期限

生产后 2 年内

iPS 细胞培养能力

添加 0.5 μg/cm² 到培养容器中，可用于 iPS 细胞的维持培养

iMatrix-511

自生产后两年内

iMatrix-511silk

自生产后两年内

iMatrix-511MG

自生产后两年内

人多能干细胞（hPSC）的确立

Miyazaki et al. Nat. Commun.3:1236, (2012)

证实用作hPSC的培养基质的有效性

Nakagawa et al. Sci. Rep. 4:3594, (2014)

确立医疗等级的hPSC

Takashima et al. Cell.158(6):1254-69, (2014)

促进向hPSC的基质状态的转移

Miyazaki et al. Sci. Rep.7:41165, (2017)

采用无需涂层操作的添加法培养hPSC

Sekine et al. Stem Cell Res.24:40-43, (2017)

确立疾病特异性的hPSC

Tan et al. Stem Cell Res. 24:12-15, (2017)

Ishida et al. Sci. Rep. 8(1), 310, (2018)

利用hPSC的基因编辑建立遗传性疾病模型

Kim et al. Nature Communications, 9(1), 939, (2018)

Sakai-Takemura et al. Sci. Rep, 8, 6555, (2018)

悬浮培养由hPSC分化的肌肉前体细胞

由hPSC分化衍生的细胞

Doi et al. Stem Cell Reports. 2(3):337-50, (2014)

多巴胺产生神经元

Ishikawa et al. Hum. Mol. Genet.25(23):

5188-5197, (2016)

Nishimura et al. Stem Cell Reports.6(4):

511-524, (2016)

Samata et al. Nat. Commun. 7:13097, (2016)

Kikuchi et al. Nature. 548(7669):592-596, 　　(2017)

Morizane et al. Nat. Commun.8(1):385, (2017)

Kikuchi et al. J. Neurosci. Res.95(9):1829-37, (2017)

Goparaju et al. Sci. Rep. 7:42367, (2017)

运动神经元

Burridge et al. Nat. Methods.11(8):855-60, 　  (2014)

心肌细胞

Sougawa et al. Sci. Rep,8(1), 3726, (2018)

Yamauchi et al. BBRC, 495(1), 1278-1284, 　    (2018)

心室肌细胞

Akiyama et al. Sci. Rep, 8(1), 1189, (2018)

骨骼肌细胞

Saito et al. Stem Cell Res Ther, 9(1), 12, (2018)

成骨细胞

Uchimura et al. Stem cell research, 25, 98-106, (2017)

成肌细胞

Hayashi et al. Nature.531(7594):376-80, (2016)

视觉细胞

Hayashi et al. Nat. Protoc.12(4):683-696, (2017)

角膜上皮细胞

Takayama et al. BBRC. 474(1):91-96, (2016)

胆管上皮细胞

Takayama et al. Hepatol Commun,1(10), 1058-1069, (2017)

肝实质细胞

Takayama et al. Biomaterials, (2018)

Takebe et al. Cell Reports, 21(10), 2661-2670, (2017)

肝细胞

Tan et al. Stem Cell Reports, 11:1-11, (2018)

Camp et al. Nature. 546(7659):533-38, (2017)

定形内胚层细胞

Zhang et al. Stem Cell Reports, 10(2), 1–14, (2018)

后内胚层前体细胞

Tanigawa et al. Cell reports, 15(4), 801-813, (2016)

肾单位前体细胞（胎肾细胞）

Musah et al. Nat.Biomed.Eng.1:0069, (2017)

肾小球上皮细胞

Musah et al. Nature protocols,13(7):1662, 　  (2018)

Mae et al. BBRC, 495(1), 954-961, (2018)

输尿管芽组织

Oshima et al. BBRC, 497(2), 719-725, (2018)

血细胞・血管内皮常见前体细胞

Taguchi et al. Cell Stem Cell, 21, (2017) 

*培养hPSC用于分化肾单位前体细胞（胎肾细胞）

Kawamura et al. Stem Cell Reports.6(3):312-20,(2016)

*培养hPSC用于分化心肌细胞

Sasaki et al. Cell Stem Cell.17(2):178-94, (2015)

*培养hPSC用于分化生殖细胞

Kojima et al. Cell Stem Cell.21(4):517-532, 　  (2017)

Furuta et al. PLoS One. 9(12):e112291, (2014)

*培养hPSC用于分化间充质细胞

人原代细胞的培养

Okumura et al. Invest. Ophth. Vis. Sci.56(5):2933-42, (2015)

人角膜内皮细胞

Hongo et al. Invest. Ophth. Vis. Sci.58(9):3325-34, (2017)

Polisetti et al. Sci. Rep.7(1):5152, (2017)

人角膜边缘上皮前体细胞

Ishii et al. Stem Cell Reports, 10, 1-15, (2018)

卫星细胞

层粘连蛋白-整合素相互作用的分子机制

Ido et al. J. Biol. Chem. 282(15): 11144-54, 　  (2007)

Ido et al. J. Biol. Chem.283(42): 28149-57, 　  (2008)

Taniguchi et al. J. Biol. Chem. 284(12): 7820-31, (2009)

Taniguchi et al. BBRC.487(3): 525-531, (2017)

Takizawa et al. Sci Adv.3(9) :e1701497, (2017)

[2]

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of human induced pluripotent stem cells into mature kidney podocytes and establishment of a 

lomerulus Chip. Nature protocols, 13(7), 1662, (2018)

[3]

Ishii, K., Sakurai, H., Suzuki, N., Mabuchi, Y., Sekiya, I., Sekiguchi, K., Akazawa, C.. Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell 

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[4]

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Takayama, K., Hagihara, Y., Toba, Y., Sekiguchi, K., Sakurai, F., Mizuguchi, H.. Enrichment of high-

functioning human iPS cell-derived hepatocyte-like cells for pharmaceutical research. 

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Sakamoto, T., Yamaguchi, A., Sueishi, K., Azuma, T.. Targeted reversion of induced pluripotent 

stem cells from patients with human cleidocranial dysplasia improves bone regeneration in a rat 

calvarial bone defect model. Stem Cell Research & Therapy, 9(1), 12, (2018)

[8]

Yamauchi, K., Li, J., Morikawa, K., Liu, L., Shirayoshi, Y., Nakatsuji, N., Elliott, A. D., Hisatome, I., 

Suemori, H..Isolation and characterization of ventricular-like cells derived from NKX2-5 eGFP/w 

and MLC2v mCherry/w double knock-in human pluripotent stem cells. Biochemical and 

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[10]

Kagihiro, M., Fukumori, K., Aoki, T., Ungkulpasvich, U., Mizutani, M., Viravaidya-Pasuwat, K.,& 

Kino-oka, M.. Kinetic analysis of cell decay during the filling process: Application to lot size 

determination in manufacturing systems for human induced pluripotent and mesenchymal stem cells. Biochemical Engineering Journal, 131, 31-38, (2018)

[11]

Zhang, R. R., Koido, M., Tadokoro, T., Ouchi, R., Matsuno, T., Ueno, Y., Sekine, K., Takebe, T., 

Taniguchi, H.. Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of 

Forming Gut and Liver Organoids. Stem Cell Reports, 10(2), 1?14, (2018)

[12]

Oshima, K., Saiki, N., Tanaka, M., Imamura, H., Niwa, A., Tanimura, A., Nagahashi, A., Hirayama, A., Okitac, K., Hotta, A., Kitayama, S., Osawa, M., Kaneko, S., Watanabe, A., Asaka, I., Fujibuchi, W., 

Imai, K., Yabe, H., Kamachi, Y., Hara, J., Kojima, S., Tomita, M., Soga, T., Noma, T., Nonoyama, S., 

Nakahata, T., Saito, MK.. Human AK2 links intracellular bioenergetic redistribution to the fate of 

hematopoietic progenitors. Biochemical and Biophysical Research Communications, 497(2), 719-

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Sougawa, N., Miyagawa, S., Fukushima, S., Kawamura, A., Yokoyama, J., Ito, E., Harada, A., 

Okimoto, K., Mochisuki-Oda, N., Saito, A., Sawa, Y.. Immunologic targeting of CD30 eliminates 

tumourigenic human pluripotent stem cells, allowing safer clinical application of hiPSC-based cell therapy. Scientific Reports, 8(1), 3726, (2018)

[14]

Yasuda, S. Y., Ikeda, T., Shahsavarani, H., Yoshida, N., Nayer, B., Hino, M., Vartak-Sharma, N.,

Suemori, H., Hasegawa, K.. Chemically defined and growth-factor-free culture system for the 

expansion and derivation of human pluripotent stem cells. Nature Biomedical Engineering, 2(3), 

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Sekiguchi, K., Quantock, A. J., Nishida, K. . Coordinated generation of multiple ocular-like cell 

lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nature 

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[17]

Kikuchi, T., Morizane, A., Okita, K., Nakagawa, M., Yamakado, H., Inoue, H., Takahashi, R., 

Takahashi, J. . Idiopathic Parkinson's disease patient‐derived induced pluripotent stem cells 

function as midbrain dopaminergic neurons in rodent brains. Journal of Neuroscience Research, 

95(9),1829-37, (2017)

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Miyazaki, T., Isobe, T., Nakatsuji, N., & Suemori, H. . Efficient Adhesion Culture of Human 

Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports, 7

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Matsushita, M., Sakota, M., Kimura, H., Yuzaki, M., Shigeru B. H. Ko & Minoru S. H. Ko. . Rapid 

differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding 

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Ingber, F. D. . Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute 

kidney glomerular-capillary-wall function on a chip. Nature Biomedical Engineering, 1 (0069), 

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Camp, J. G., Sekine, K., Gerber, T., Loeffler-Wirth, H., Binder, H., Gac, M., Kanton, S., Kageyama, J., Damm, G., Seehofer, D., Belicova, L., Barsacchi, M., Barsacchi, R., Okuda, R., Yoshizawa, E., Kimura, M., Ayabe, H., Taniguchi, H., Takebe, T., & Belicova, L.. Multilineage communication regulates 

human liver bud development from pluripotency. Nature, 546, 533-538, (2017)

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Polisetti, N., Sorokin, L., Okumura, N., Koizumi, N., Kinoshita, S., Kruse, F. E., and Schlotzer-

Schrehardt, U. Laminin-511 and-521-based matrices for efficient ex vivo-expansion of human 

limbal epithelial progenitor cells. Scientific Reports, 7, 5152, (2017)

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Hongo, A., Okumura, N., Nakahara, M., Kay, E. P., & Koizumi, N.. The Effect of a p38 Mitogen-

Activated Protein Kinase Inhibitor on Cellular Senescence of Cultivated Human Corneal 

Endothelial CellsEffect of a p38 MAPK Inhibitor on Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science, 58(9), 3325-3334, (2017)

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Taniguchi, Y., Li, S., Takizawa, M., Oonishi, E., Toga, J., Yagi, E., & Sekiguchi, K. Probing the acidic 

residue within the integrin binding site of laminin-511 that interacts with the metal ion-

dependent adhesion site of α6β1 integrin. Biochemical and Biophysical Research 

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patient with familial idiopathic basal ganglia calcification (IBGC) caused by a variant in SLC20A2 

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leucine-rich glioma inactivated 1 (LGI1). Stem Cell Research, (2017)

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Sato-Nishiuchi, R., Li, S., Ebisu, F., Sekiguchi, K.. Recombinant laminin fragments endowed with 

collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen 

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Kikuchi, T., Morizane, A., Doi, D., Magotani, H., Onoe, H., Hayashi, T., Mizuma, H., Takara, S., 

Takahashi, R., Inoue, H., Morita, S., Yamamoto, M., Okita, K., Nakagawa, M., Parmar, M., Takahashi, J.. Human iPS cell-derived dopaminergic neurons function in a primate Parkinson's disease 

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Mechanistic basis for the recognition of laminin-511 by α6β1 integrin. Science Advances, 3(9), 

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Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem 

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and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells. Cell 

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Uchimura, T., Otomo, J., Sato, M., Sakurai, H.. A human iPS cell myogenic differentiation system 

permitting high-throughput drug screening. Stem cell research, 25, 98-106, (2017)

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Sougawa, N., Miyagawa, S., Fukushima, S., Saito, A., Yokoyama, J., Kitahara, M., Harada, A., Sato-

Nishiuchi, R., Sekiguchi, K., Sawa, Y.. Novel Stem Cell Niches Laminin 511 Promotes Functional 

Angiogenesis Through Enhanced Stem Cell Homing by Modulating" Stem Cell Beds" in the Failed Heart.Circulation, 136(1), A15587, (2017)

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Samata, B., Doi, D., Nishimura, K., Kikuchi, T., Watanabe, A., Sakamoto, Y., Kakuta, J., Ono, Y.,& 

Takahashi, J.. Purification of functional human ES and iPSC-derived midbrain dopaminergic 

progenitors using LRTM1. Nature Communications, 7(13097), 1-11, (2016)

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Hayashi, R., Ishikawa, Y., Sasamoto, Y., Katori, R., Nomura, N., Ichikawa, T., Araki, S., Soma, T., 

Kawasaki, S., Sekiguchi, K., Tsujikawa, M., Nishida, K., & Quantock, A. J.. Co-ordinated ocular 

development from human iPS cells and recovery of corneal function. Nature, 531(7594), 376-380, (2016)

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Matsuno, K., Mae, S. I., Okada, C., Nakamura, M., Watanabe, A., Toyoda, T., Uchida, E., Osafune, K.. Redefining definitive endoderm subtypes by robust induction of human induced pluripotent 

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[40]

Nishimura, K., Doi, D., Samata, B., Murayama, S., Tahara, T., Onoe, H., & Takahashi, J.. Estradiol 

Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal 

Circuits via Activation of Integrin α5β1. Stem cell reports, 6(4), 511-524, (2016)

[41]

Takayama, K., Mitani, S., Nagamoto, Y., Sakurai, F., Tachibana, M., Taniguchi, Y., Sekiguchi,K., 

Mizuguchi, H.. Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474(1), 91-96, (2016)

[42]

Kawamura, T., Miyagawa, S., Fukushima, S., Maeda, A., Kashiyama, N., Kawamura, A., Miki, K., 

Okita, K., Yoshida, Y., Shiina, T., Ogasawara, K., Miyagawa, S., Toda, K., Okuyama, H., Sawa,Y.. 

Cardiomyocytes derived from MHC-homozygous induced pluripotent stem cells exhibit reduced allogeneic immunogenicity in MHC-matched non-human primates. Stem cell reports, 6(3), 312-

320, (2016).

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Tanigawa, S., Taguchi, A., Sharma, N., Perantoni, A. O., & Nishinakamura, R.. Selective in vitro 

propagation of nephron progenitors derived from embryos and pluripotent stem cells. Cell 

reports, 15(4), 801-813, (2016)

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Okumura, N., Kakutani, K., Numata, R., Nakahara, M., Schlotzer-Schrehardt, U., Kruse, F., Kinoshita. K., Koizumi, N.. Laminin-511 and-521 Enable Efficient In Vitro Expansion of Human Corneal 

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Woltjen, K., Nakagawa, M., Yamamoto, T., Takahashi, K., Yamanaka, S., Saitou, M.. Robust in vitro 

induction of human germ cell fate from pluripotent stem cells. Cell stem cell, 17(2), 178-194, 

(2015)

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Takahashi, J., Nishizawa, M., Yoshida, Y., Toyoda, T., Osafune, K., Sekiguchi, K., & Yamanaka, S. . A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem 

cells. Scientific reports, 4(3594), 1-7, (2014)

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Doi, D., Samata, B., Katsukawa, M., Kikuchi, T., Morizane, A., Ono, Y., Sekiguchi, K., Nakagawa, M., Parmar, M., Takahashi, J.. Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation. Stem cell reports, 2(3), 337-350, (2014)

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Takashima, Y., Guo, G., Loos, R., Nichols, J., Ficz, G., Krueger, F., Oxley, D., Santos, F., Clarke, J., 

Mansfield, W., Reik, W., Bertone, P., Smith, A.. Resetting transcription factor control circuitry 

toward ground-state pluripotency in human. Cell, 158(6), 1254-1269, (2014)

[49]

Fukuta, M., Nakai, Y., Kirino, K., Nakagawa, M., Sekiguchi, K., Nagata, S., Matsumoto, Y., 

Yamamoto, T., Umeda, K., Heike, T., Okumura, N., Koizumi, N., Sato, T., Nakahata, T., Saito, M., 

Otsuka, T., Kinoshita, S., Ueno, M., Ikeya, M., Toguchida, J. . Derivation of mesenchymal stromal 

cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media. PloS one, 9(12), e112291, (2014)

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