重组人MEGACD40L® 蛋白（可溶）
用于免疫反应中协同刺激激活的高活性、高纯度CD40L蛋白质。
◆特点
● 高活性MEGACD40L® 低聚物模拟体内膜辅助CD40L聚合和激活而无需增强剂
● 高纯度，低内毒素水平
● 丰富的生产经验保证产品批次间一致性和可靠性
● 优化的配方以增强稳定性并提高活性
MEGACD40L是一种高活性蛋白，是两个三聚体CD40配体分子通过Adiponectin/ACRP30/AdipoQ的胶原域人工连接而成。在体内，这种蛋白质能有效的模拟CD40L的天然膜辅助聚合。可简单有效的替代 [CD40L+增强剂]组合（产品编号：ALX-850-064）。
◆产品规格
别名 | CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless |
分子量 | ~38 kDa |
来源 | CHO细胞表达。人CD40L（CD154）（aa 116-261）与小鼠ACRP30headless（aa 18-111）N端以及一个 FLAG® -tag融合。 |
UniProt ID | P29965 (human CD40L), Q60994 (mouse Adiponectin) |
浓度 | 复溶后0.1 mg/mL |
产品类型 | 冻干，含PBS |
纯度 | ≥90%（SDS-PAGE） |
纯化细节 | 多步层析纯化 |
内毒素含量 | ≤0.01EU/μg 纯化蛋白（LAL 测试；Cape Cod Associates） |
生物活性 | 与人CD40结合。B细胞激活（已证明存在CD86剂量依赖性上调） |
应用 | 可用于调节免疫和炎症反应，作为免疫治疗的临床前研究的一种蛋白质工具，用来监测病人接受化疗的过程 以及模拟自体免疫反应表型。 |
配制 | 用100 μL的无菌水溶解。以含5%胎牛血清的培养基进行进一步稀释。 |
运输 | 冰袋运输 |
长期储存 | -20℃ |
使用/稳定性 | 收货后可稳定储存在-20℃至少6个月 |
处理 | 避免反复冻融。复溶后，分装成小份储存于-20℃。 |
技术信息/产品说明 | FLAG是Sigma-Aldrich Co.的注册商标。 |
◆应用实例
MEGACD40L® (产品编号：ALX-522-110)激活B淋巴细胞。方法：外周血单核细胞（PBMCs）37℃、5%CO2 在48孔板（1×106 细胞/孔）中培养48小时。每孔包含200 μL已梯度稀释的MEGACD40L® 无血清测试培养基。处理后，清洗细胞，用小鼠anti-human CD19-PE和小鼠anti-humanCD86-APC进行双重染色，然后用流式细胞术进行分析。所示数据为在不同的MEGACD40L® 浓度下，共染色为CD19+ 和CD86+ B细胞的百分数。
重组人MEGACD40L®（可溶）（产品编号：ALX-522-110）结构示意图
PBMCs（外周血单核细胞）37℃、5%CO2 在48孔板（1×106细胞/孔）中培养48小时，每孔包含200 μL已梯度稀释的MEGACD40L®（产品编号：ALX-522-110）无血清测试培养基、CD40L(产品编号：ALX-522-015)和CD40L+2 μg/mL 增强剂（产品编号：ALX-804-034）。处理后，用PBS+1%BSA清洗细胞3次。用小鼠anti-human CD19（PE偶联）和小鼠anti-humanCD86（APC偶联）进行双重染色，4℃，2小时。用PBS+1%BSA清洗细胞3次，用PBS重悬。样品用BD Facs Calibur 流式细胞分析仪进行分析。所示数据为在不同的浓度下CD86+B细胞百分数/CD19+B细胞百分数。
1000 ng/mL、7个连续批次的MEGACD40L® 用于B细胞激活实验数据
通过在指定的温度下储存蛋白2个月来进行MEGACD40L® 稳定性测试。用B细胞激活实验来进行蛋白活性测试。结果显示在严苛的储存条件下MEGACD40L® 仍能保持较好的活性。
◆相关产品
产品编号 | 产品名称 | 包装 | 产品描述 |
MEGACD40L (soluble) (mouse), (recombinant) | 10 μg | 用于免疫反应中协同激活活性的高活性、高纯度CD40L蛋白。 | |
CD40L, soluble (human) (recombinant) set | 1 set | 配体+增强剂用于提高稳定性和增强免疫活性。 | |
CD40L (human) monoclonal antibody (blocking) (MK13A4) | 100 μg | 流式细胞术，FUNC | |
CD40L (soluble) (human), (recombinant) | 6×10 μg | 增加对B细胞的刺激。 | |
2×10 μg | |||
10 μg |
参考文献
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| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
| ALX-522-110-C010 | MEGACD40L (soluble) (human), (recombinant) 重组人Mega CD40L（可溶） |
10 μg | - | - |
| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
| ALX-522-120-C010 | MEGACD40L® Protein (soluble) (mouse), (recombinant) 重组小鼠MEGACD40L可溶性蛋白 |
10 µg | - | - |
| ALX-850-064-KI01 | CD40L, soluble (human) (recombinant) set CD40L, 可溶性 （人） （重组） 套装 |
1 Set | - | - |
| ALX-805-037-C100 | CD40L (human) monoclonal antibody (blocking) (MK13A4) CD40L （人）, 单抗 （封闭作用） （MK13A4） |
100 µg | - | - |
| ALX-522-015-6010 | CD40L (soluble) (human), (recombinant) CD40L （可溶性） （人）, （重组） |
6×10 µg | - | - |
| ALX-522-015-2010 | CD40L (soluble) (human), (recombinant) CD40L （可溶性） （人）, （重组） |
2×10 µg | - | - |
| ALX-522-015-C010 | CD40L (soluble) (human), (recombinant) CD40L（可溶性）（人），（重组） |
10 µg | - | - |
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