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鼠源Iba1抗体,无标签,单克隆抗体(NCNP24)
Anti Iba1, Monoclonal Antibody (NCNP24)

新品速递图wako.jpg小胶质标记

鼠源无标签抗Iba1,单克隆抗体(NCNP24)




  Iba1是在小胶质细胞和巨噬细胞中高度表达的蛋白质,分子量约17 kDa。 此蛋白质通常称为中枢神经系统中的小胶质细胞标记物。

  最近,小胶质细胞已被广泛研究,其负责中枢神经系统中的免疫功能,并且与各种疾病相关,如神经变性疾病,精神错乱,脑肿瘤和感染。

2314902585681122.jpg




类别:Monoclonal

应用物种:IHC(小鼠、大鼠和狨猴冰冻切片,DABlocking)(1:500-2,000),

应用物种IHC(大鼠冰冻切片,fluorescent)(1:100)



◆特点


● 免疫组化评估

● 用于多色免疫染色

● 具有高特异性和低背景的单克隆抗体



应用 - 免疫组织化学染色


2314902585771205.jpg


使用抗Iba1,单克隆抗体(FUJIFILM Wako产品编号:012-26723)小胶质细胞的定位信号清晰,与抗Iba1,兔多克隆抗体的效果相同(FUJIFILM Wako产品编号:019-19741、019-19741)。



实验条件


● 样品:7周龄大鼠,7周龄小鼠或成年狨猴的大脑皮质

● 切片:50 μm冰冻切片(小鼠和大鼠),40 μm冰冻切片(狨猴)

● 染色法:ABC法+DAB染色

● 抗体浓度= 1:500


数据提供


Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan. 


抗体信息


抗Iba1,单克隆抗体(NCNP24)

抗原

合成肽(Iba1的C末端)

克隆号

NCNP24

子类

小鼠IgG1

浓度

1.3 mg/mL

Buffer缓冲液

50%甘油/ TBS,0.05%叠氮化钠

物种反应性

小鼠,大鼠,狨猴

应用

免疫组织化学(冷冻切片)(1:500-2,000)



欲了解相关产品信息请点击文字:

兔源Iba1抗体,有标签

兔源Iba1抗体,无标签


欲了解相关知识请点击文字:

巨噬细胞/小胶质细胞特异性蛋白抗体Iba1的应用

小胶质细胞研究动向与新型Iba1标签抗体


欲了解相关资料请点击文字:

Wako神经生物学抗体清单

巨噬细胞/小胶质细胞Iba1抗体


◆相关产品


产品编号

产品名称

中文名称

包装

储存条件

016-26461

Anti Iba1, Rabbit, Biotin-conjugated

抗Iba1,兔,生物素缀合

100 μL

保存在2-10℃

013-26471

Anti Iba1, Rabbit, Red Fluorochrome   (635)-conjugated

抗Iba1,兔,红色荧光染料(635)结合

100 μL

 


◆相关资料


QQ图片20200427144555.pngQQ图片20200427104915.png2914907606046414.jpg
Iba1鼠源无标签Iba1抗体概述

012-26723,016-26721

说明书

QQ图片20200415115500.pngQQ图片20200427101936.png
神经生物学产品系列Iba1抗体系列



参考文献



1.

Moghaddam, B. (2018). Omega-3 Polyunsaturated Fatty Acid Status, Microglial Activation, Stress Resilience, and Cognitive Performance. Oregon Health and Science University Portland United States.


2.

Li, K., Zhou, H., Zhan, L., Shi, Z., Sun, W., Liu, D., ... & Xu, E. (2018). Hypoxic preconditioning maintains GLT-1 against transient global cerebral ischemia through upregulating Cx43 and inhibiting c-Src. Frontiers in molecular neuroscience, 11.


3.

Gasparotto, J., Ribeiro, C. T., da Rosa-Silva, H. T., Bortolin, R. C., Rabelo, T. K., Peixoto, D. O., ... & Gelain, D. P. (2019). Systemic inflammation changes the site of RAGE expression from endothelial cells to neurons in different brain areas. Molecular neurobiology, 56(5), 3079-3089.


4.

Wanrooy, B. J., Kumar, K. P., Wen, S. W., Qin, C. X., Ritchie, R. H., & Wong, C. H. (2018). Distinct contributions of hyperglycemia and high-fat feeding in metabolic syndrome-induced neuroinflammation. Journal of neuroinflammation, 15(1), 293.


5.

Velazquez, R., Ferreira, E., Winslow, W., Dave, N., Piras, I. S., Naymik, M., ... & Oddo, S. (2019). Maternal choline supplementation ameliorates Alzheimer’s disease pathology by reducing brain homocysteine levels across multiple generations. Molecular psychiatry, 1.


6.

Sanchez-Ruiz, M., Brunn, A., Montesinos-Rongen, M., Rudroff, C., Hartmann, M., Schlüter, D., ... & Deckert, M. (2019). Enteric Murine Ganglionitis Induced by Autoimmune CD8 T Cells Mimics Human Gastrointestinal Dysmotility. The American journal of pathology, 189(3), 540-551.


7.

Cougnoux, A., Fellmeth, M., Gu, T., Davidson, C. D., Gibson, A. L., Pavan, W. J., & Porter, F. D. (2019). Maternal immune activation modifies the course of Niemann-pick disease, type C1 in a gender specific manner. Molecular genetics and metabolism.


8.

Groves, A., Kihara, Y., Jonnalagadda, D., Rivera, R., Kennedy, G., Mayford, M., & Chun, J. (2018). A functionally defined in vivo astrocyte population identified by c-Fos activation in a mouse model of multiple sclerosis modulated by S1P signaling: immediate-early astrocytes (ieAstrocytes). eNeuro, 5(5).


9.

Cai, W., Wang, J., Hu, M., Chen, X., Lu, Z., Bellanti, J. A., & Zheng, S. G. (2019). All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling. Journal of neuroinflammation, 16(1), 175.


10.

Feng, T., Yamashita, T., Shang, J., Shi, X., Nakano, Y., Morihara, R., ... & Matsumoto, N. (2019). Clinical and Pathological Benefits of Edaravone for Alzheimer’s Disease with Chronic Cerebral Hypoperfusion in a Novel Mouse Model. Journal of Alzheimer's Disease, (Preprint), 1-13.


11.

Chen, Y. J., Nguyen, H. M., Maezawa, I., Jin, L. W., & Wulff, H. (2018). Inhibition of the potassium channel Kv1. 3 reduces infarction and inflammation in ischemic stroke. Annals of clinical and translational neurology, 5(2), 147-161.


12.

Michaelis, K. A., Norgard, M. A., Levasseur, P. R., Olson, B., Burfeind, K. G., Buenafe, A. C., ... & Marks, D. L. (2019). Persistent Toll-like receptor 7 stimulation induces behavioral and molecular innate immune tolerance. Brain, behavior, and immunity, 82, 338-353.


13.

Surrao, D. C., Greferath, U., Chau, Y. Q., Skabo, S. J., Huynh, M., Shelat, K. J., ... & Liu, Q. (2017). Design, development and characterization of synthetic Bruch’s membranes. Acta biomaterialia, 64, 357-376.



产品编号 产品名称 产品规格 产品等级
012-26723 Anti Iba1, Monoclonal Antibody (NCNP24)
抗Iba1,单克隆抗体(NCNP24)
10 μL
016-26721 Anti Iba1, Monoclonal Antibody (NCNP24)
抗Iba1,单克隆抗体(NCNP24)
50 μL
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