LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1（OVA-IgG1）ELISA试剂盒
IgG是血清中免疫球蛋白中含量最高的二次免疫应答主要抗体。占据三分之二IgG的是具有γ1的H链，分子量146 kDa的IgG1。以OVA（卵清蛋白）作为特殊化抗原，通过简化测定抗OVA- IgG1抗体值来阐明小鼠免疫系统作用机理。
小鼠卵清蛋白特异性免疫球蛋白G1（OVA-IgG1）ELISA试剂盒是仅特异性测定Anit-OVA-IgG1的试剂盒。
◆特点
● 测定时间短（总反应时间：1小时50分钟）
● 微量样本即可测定。
● 使用无害的防腐剂。
● 全部试剂为溶液即用类型。
● 高测定精度和高重复性。
● 操作简便，无特殊前处理。
◆试剂盒组成
组成品 | 状态 | 包装 |
OVA包被96孔板（干燥板） | 清洗后使用 | 96 wells（8×12）／1个 |
标准溶液（Anti OVA- IgG1:1,200U/mL）（单抗） | 稀释后使用 | 100 μL/1瓶 |
缓冲液 | 直接使用 | 60 mL/1瓶 |
生物素结合抗小鼠IgG1抗体（单抗） | 稀释后使用 | 200 μL/1瓶 |
过氧化物酶·抗生素结合物 | 稀释后使用 | 200 μL/1瓶 |
显色液（TMB） | 直接使用 | 12 mL/1瓶 |
终止液（1M H2SO4） ※小心轻放 | 直接使用 | 12 mL/1瓶 |
浓缩清洗液（10×） | 稀释后使用 | 100 mL/1瓶 |
孔板密封膜 | 3个 | |
产品说明书 | 1本 |
◆样本信息
● 小鼠血清·血浆
● 10 μL/well（稀释样本）
※ 样本必须用附带的缓冲液稀释100倍以上。
◆测定范围
1.88～120 mU/mL（标准曲线范围）
（本试剂盒中1 U/mL定义为抗原结合常数（Ka）为6.9×107 M-1的抗体160ng/mL）
◆实验数据
精度测试（组内变异）
样本 | A | B |
1 | 105 | 18.2 |
2 | 100 | 16.9 |
3 | 96.2 | 16.7 |
4 | 100 | 17.4 |
5 | 106 | 17.4 |
Mean | 101 | 17.3 |
SD | 4.0 | 0.59 |
CV（%） | 4.0 | 3.4 |
单位：mU/mL
重复性测试（组间变异）
测定日/样本 | C | D | E |
0天 | 60.1 | 15.0 | 3.75 |
1天 | 58.5 | 14.7 | 3.70 |
2天 | 59.9 | 15.3 | 3.83 |
3天 | 61.4 | 15.7 | 3.82 |
Mean | 60.0 | 15.2 | 3.77 |
SD | 1.2 | 0.44 | 0.06 |
CV（%） | 2.0 | 2.9 | 1.6 |
单位：mU/mL
加标回收测试
样本F
添加量 | 实测值 | 回收量 | 回收率（%） |
0.00 | 8.86 | — | — |
7.29 | 15.8 | 6.94 | 95.2 |
11.0 | 19.5 | 10.6 | 96.4 |
14.6 | 23.0 | 14.1 | 96.6 |
单位：mU/mL，n=3
样本G
添加量 | 实测值 | 回收量 | 回收率（%） |
0.00 | 51.5 | — | — |
26.9 | 76.8 | 25.3 | 97.1 |
31.4 | 80.6 | 29.1 | 92.5 |
47.1 | 100 | 48.5 | 103 |
单位：mU/mL，n=3
稀释直线性测试
2个血清样本连续用稀释缓冲液稀释3个梯度测定结果，直线回归值R2=0.9994~0.9998
参考文献
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53. | Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008 |
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55. | Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008 |
| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
| 630-07669 | （AKRIE-040）小鼠卵清蛋白特异性免疫球蛋白G1（OVA-IgG1） ELISA试剂盒 LBIS® OVA-IgG1 Mouse |
96 tests | - | - |
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