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StemAb™ 干细胞用碱性磷酸酶染色试剂盒

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StemAb™ 干细胞用碱性磷酸酶染色试剂盒



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碱性磷酸酶(AP)在多能干细胞中高水平表达。 AP 在碱性条件下使核苷酸、蛋白和生物碱去磷酸化。 染色后,未分化细胞呈现红色或紫色,而分化细胞呈现无色。

胚胎干细胞(ES)和诱导多能干细胞(iPS)的未分化状态可以通过高水平的碱性磷酸酶(AP)表达来表征,连同其他表面标志物的表达,表明未分化细胞具有自我更新潜力。AP 是一种水解酶,负责在碱性条件下使核苷酸,蛋白和生物碱去磷酸化。 当使用 AP 染色试剂盒对固定的 ES 或 iPS 细胞染色时,未分化细胞呈现红色或紫色,而分化细胞呈现无色。



◆试剂盒组成


固定溶液:  25 mL 

AP 染色溶液 A:10 mL
AP 染色溶液 B:10 mL

AP 染色溶液 C:10 mL



储存和稳定性


● 在 4°C 储存, 不可冷冻储存。到货后妥善存储可稳定 6 个月。




质量控制


StemAb™ 干细胞用碱性磷酸酶染色试剂盒已在人 iPS 细胞和小鼠 ES 细胞上进行功能测试,确保产品质量。



◆操作步骤


StemAb Alkaline Phosphatase Staining Kit II



※ 本页面产品仅供研究用,研究以外不可使用。

参考文献


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◆其他相关资料


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Chung CY; Lo PHY; Lee KKH. Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage. Biomedicines 8:397 (2020).全文


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Choi NY; Bang JS; Park YS; Lee M; Hwange HS; Ko K; Myung SC; Tapia N; Schöler HR; Kim GJ; Ko K. Generation of human androgenetic induced pluripotent stem cells. Sci Rep 10:3614 (2020). 全文


 9.

Ha M; Athirasala A; Tahayeri A; Menezes PP; Bertassoni LE. Micropatterned hydrogels and cell alignment enhance the odontogenic potential of stem cells from apical papilla in-vitro. Dental Materials in press:doi.org/10.1016/j.dental.2019.10.013 (2019).全文


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Ge N; Liu M; Krawczyz J; McInerney V; Galvin J; Shen S; O'Brien T; Prendiville T. Generation of eight human induced pluripotent stem cell (iPSC) lines from familial Long QT Syndrome type 1 (LQT1) patients carrying KCNQ1 c.1697C>A mutation (NUIGi005-A, NUIGi005-B, NUIGi005-C, NUIGi006-A, NUIGi006-B, NUIGi006-C, NUIGi007-A, and NUIGi007-B). Stem Cell Res in press:https://doi.org/10.1016/j.scr.2019.101502 (2019).全文


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产品列表
产品编号 产品名称 产品规格 产品等级 备注
00-0055 Stemgent Alkaline Phosphatase Staining Kit II (50 assays) 
Stemgent碱性磷酸酶染色试剂盒II
50 assays

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