StemAb™ 干细胞用碱性磷酸酶染色试剂盒
碱性磷酸酶(AP)在多能干细胞中高水平表达。 AP 在碱性条件下使核苷酸、蛋白和生物碱去磷酸化。 染色后,未分化细胞呈现红色或紫色,而分化细胞呈现无色。
胚胎干细胞(ES)和诱导多能干细胞(iPS)的未分化状态可以通过高水平的碱性磷酸酶(AP)表达来表征,连同其他表面标志物的表达,表明未分化细胞具有自我更新潜力。AP 是一种水解酶,负责在碱性条件下使核苷酸,蛋白和生物碱去磷酸化。 当使用 AP 染色试剂盒对固定的 ES 或 iPS 细胞染色时,未分化细胞呈现红色或紫色,而分化细胞呈现无色。
◆试剂盒组成
固定溶液: 25 mL
AP 染色溶液 A:10 mL
AP 染色溶液 B:10 mL
AP 染色溶液 C:10 mL
◆储存和稳定性
● 在 4°C 储存, 不可冷冻储存。到货后妥善存储可稳定 6 个月。
◆质量控制
StemAb™ 干细胞用碱性磷酸酶染色试剂盒已在人 iPS 细胞和小鼠 ES 细胞上进行功能测试,确保产品质量。
◆操作步骤
StemAb Alkaline Phosphatase Staining Kit II
※ 本页面产品仅供研究用,研究以外不可使用。
参考文献
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◆其他相关资料
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产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
00-0055 | Stemgent Alkaline Phosphatase Staining Kit II (50 assays) Stemgent碱性磷酸酶染色试剂盒II |
50 assays | - | - |
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