Phos-tag™ SDS-PAGE相关产品
◆Reagents for Phos-tag™ SDS-PAGE gel preparation
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
30 w/v% Acrylamide/Bis Mixed solution(29:1) | 500 mL | 015-25635 | Ready-to-use"Solution A".30%T, 3.3%C |
Agarose H（High-strength type） | 1 g | High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 - 1% and a separation range of 1 – 200 kbp. | |
10 g | |||
25 g | |||
10% SDS Solution | 100 mL | Ready-to-Use "Solution D" | |
500 mL | |||
Manganese(Ⅱ) Chloride Tetrahydrate, | 25 g | for Molecular Biology Please use for preparation of "Solution F" | |
100 g | |||
Zinc Chloride | 25 g | for Molecular Biology. Please use for preparation of "Solution M" | |
Bis-Tris | 100 g | Please use for preparation of "Solution N" | |
Sodium Hydrogensulfite〈JIS Special Grade〉 | 25 g | Please use for preparation of "Solution O" | |
100 g | |||
Sodium Hydrogensulfite | 100 g | ||
MOPS | 100 g | Please use for preparation of "Solution P" | |
250 g | |||
MOPS〈For Molecular Biology〉 | 100 g | ||
500 g | 343-08245 | ||
Separating Gel Buffer Solution (x4) | 250 mL | Ready-to-Use mixed solution of“Sol. B”and“Sol. D”for preparation of Resolving Gel. Contains SDS. | |
Stacking Gel Buffer Solution (x4) | 250 mL | Ready-to-Use mixed solution of“Sol.C”and“Sol. D”for preparation of Stacking Gel. Contains SDS. | |
N,N,N',N'-Tetramethylethylenediamine (TEMED) | 25 mL | for Electrophoresis | |
10 w/v% Ammonium Peroxodisulfate Solution | 25 mL | Ready-to-Use“Solution G” |
◆Premixed Buffers
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
Running Buffer Solution (x10) | 1 L | Ready-to-Use concentrated "Solution H" | |
SDS-PAGE 10x Running Buffer | 1 L | Ready-to-Use concentrated "Solution H" | |
5 L | |||
SDS-PAGE Buffer, pH 8.5 | 5 L | Ready-to-Use "Solution H", 1×buffer. | |
Tricine Running Buffer Solution (×10) | 1 L | Composition: 0.5 M Tris/0.5 M Tricine/1% SDS | |
Sample Buffer Solution (2ME+) (×2) | 25 mL | Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol | |
Sample Buffer Solution | 25 mL | Laemmli Sample Buffer containing 3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME | |
AquaBlot™ 10×High Efficiency Transfer Buffer | 1 L | Can use it to transfer to a menbrane with high efficience. |
◆Enzyme for Dephosphorylation
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
Alkaline Phosphatase（for Biochemistry） | 50 U | Applicable to dephosphorylation of proteins | |
100 U |
◆Reagents for Staining
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
Quick CBB Plus | 250 mL | Ready-to-Use Sol. K. Fixing and destaining procedure are not required. | |
1 L | |||
Quick-CBB | 2 L | By mixing staing solution A and B, ready-to-Use Sol. K | |
Silver Stain MS Kit | 20 tests | Modified proteins by glycosilation and/or phosphorylation can be detected sub-nanogram level on the electrophoretic gel. | |
Silver Stain Kit Wako | for 10 gels | 50~100 times more sensitive than CBB method. | |
Silver Stain Ⅱ Kit Wako | for 10 gels | This kit contains Stopper, which can be adjusted the staining strength. | |
Negative Gel Stain MS Kit | 20 tests | Applicable to mass analysis and Western blot |
◆Protein Size Marker
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
WIDE-VIEW™ Prestained Protein Size Marker Ⅲ | 25 μL | A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less distorted. | |
500 μL | |||
500 μL x3 |
◆Positive Control（for confirmation of separation capacity of Phos-tag™ SDS-PAGE）
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
α-Casein, from BovineMilk, Dephosphorylated | 1 mg | Mixture of phosphylated and dephosphorylated α-casein | |
10 mg |
◆Electrophoresis Apparatus ・ Precast Gels
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
EasySeparator | 1 unit | An electrophoresis tank for SuperSep precast polyacrylamide gels. | |
SuperSep Ace, 6%, 13 wells | 10 gels | Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation. | |
SuperSep Ace, 7.5%, 13 wells | 10 gels | ||
SuperSep Ace, 7.5%, 17 wells | 10 gels | ||
SuperSep Ace, 10%, 13 wells | 10 gels | ||
SuperSep Ace, 10%, 17 wells | 10 gels | ||
SuperSep Ace, 12.5%, 13 wells | 10 gels | ||
SuperSep Ace, 12.5%, 17 wells | 10 gels | ||
SuperSep Ace, 15%, 13 wells | 10 gels | ||
SuperSep Ace, 15%, 17 wells | 10 gels |
◆Reagents for Western Blotting
Product Name | Pkg. Size | Wako Cat. No. | Use Application |
ImmunoStar LD＊ | 200 cm2 | Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate. | |
1,000 cm2 | |||
2,000 cm2 | |||
ImmunoStar Zeta＊ | 200 cm2 | Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal. | |
1,000 cm2 | |||
2,000 cm2 | |||
ImmunoStar Basic＊ | 200 cm2 | A cost-effective, stable and long-lasting product. | |
2,000 cm2 | |||
5,000 cm2 | |||
Immuno Enhancer | 2 assays | Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA | |
10 assays | |||
40 assays |
＊ : Not available for sales in the US and Europe.
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