PROTEOSTAT® PDI assay kit
蛋白二硫键异构酶检测试剂盒
PROTEOSTAT® PDI 检测试剂盒能够以稳定且高通量的方式定量检测PDI酶活性，适用于从化学库中筛选PDI抑制剂，为筛选PDI酶活性调节剂提供了一种简单、均相的检测方法。
本试剂盒在二硫苏糖醇（DTT）存在的条件下，PDI酶催化胰岛素聚集体的形成，随后与发射红色荧光的 PROTEOSTAT® PDI检测染料结合，通过监测胰岛素减少来实现对PDI酶活性的检测。
相对于使用比浊法检测PDI酶活性，基于荧光的检测法有着极大改进的信号检测窗口和检测下限，以及优越的 Z' 因子（>0.8）。检测中板内和板间CV值为3-5%。
本试剂盒中包含人重组PDI酶（EC 5.3.4.1）、杆菌肽（PDI抑制剂）以及监测PDI酶活性变化所需的全部试剂。
◆产品详情
基础信息
别称：蛋白二硫键异构酶（Protein disulfide isomerase）检测试剂盒
应用：荧光检测
储存条件：避光。避免反复冻融。
运输方式：干冰运输
长期储存温度：–80°C
特点：本试剂盒为筛选蛋白二硫键异构酶（PDI）酶活性的调节剂提供了一种简单、均相的检测方法。
UniProt ID：P07237（人）
监管状态：RUO - 仅供研究使用
技术信息/产品说明：Enzo和PROTEOSTAT®是Enzo Life Sciences, Inc. 的商标。
技术信息/产品说明：Enzo的部分产品和产品应用受美国、外国专利及正在申请的专利保护。
品质保证
（1）按照手册中的步骤， PROTEOSTAT® PDI检测试剂盒可用于检测以下样品：
（1）① 含PDI的胰岛素；② 不含PDI的胰岛素；③ 含有PDI和杆菌肽的胰岛素。
（2）Z' 因子高于0.8。
（3）PDI活性至少可被杆菌肽抑制50%。
使用/稳定性
在产品标签注明的日期前，试剂盒组分在适当的储存条件下可稳定保存。
可存放于-20°C的无霜冰箱中，或在–80°C下长期保存。
组分
● PROTEOSTAT® PDI 检测试剂
● PDI（人）（重组）
● 胰岛素（冻干）
● 杆菌肽（抑制剂对照，冻干）
● PBE 缓冲液
● 终止试剂
● DTT（10 mM）
● 去离子水
※ 本页面产品仅供研究用，研究以外不可使用。
参考文献
1. | Thioredoxin reductase is a major regulator of metabolism in leukemia cells: S. Karunanithi, et al.; Oncogene 41388 (2021), Abstract; |
2. | Molecular docking‐assisted screening reveals tannic acid as a natural protein disulphide isomerase inhibitor with antiplatelet and antithrombotic activities: L. Ren, et al.; J. Cell. Mol. Med. 24, 14257 (2020), Abstract; Full Text |
3. | PDI knockdown inhibits seizure activity in acute seizure and chronic epilepsy rat models via S-nitrosylation-independent thiolation on NMDA receptor: A.R. Jeon, et al.; Front. Cell. Neurosci. 12, 438 (2018), Application(s): Hippocampal tissue lysates, Abstract; Full Text |
4. | The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition: S. Hager, et al.; Cell Death Dis. 9, 1052 (2018), Abstract; Full Text |
5. | Tissue Factor Coagulant Activity is Regulated by the Plasma Membrane Microenvironment: Y. Yu, et al.; Thromb. Haemost. 118, 990 (2018), Application(s): In-vitro activity assay, Abstract; |
6. | Curcumin and Curcuma longa L. extract ameliorate lipid accumulation through the regulation of the endoplasmic reticulum redox and ER stress: H.Y. Lee, et al.; Sci. Rep. 7, 6513 (2017), Application(s): PDI assay of tissue lysates, Abstract; Full Text |
7. | Nox4 regulates the eNOS uncoupling process in aging endothelial cells: H.Y. Lee, et al.; Free Radic. Biol. Med. 113, 26 (2017), Application(s): PDI assay of HUVEC cell lysates, Abstract; |
8. | PDI regulates seizure activity via NMDA receptor redox in rats: J.Y. Kim, et al.; Sci. Rep. 7, 42491 (2017), Application(s): Hippocampal tissue lysates, Abstract; Full Text |
9. | Protein disulfide isomerase regulates renal AT1 receptor function and blood pressure in rats: X. Wang, et al.; Am. J. Physiol. Renal. Physiol. 313, F461 (2017), Application(s): Renal tissue lysates, Abstract; Full Text |
10. | Requirement of zinc transporter SLC39A7/ZIP7 for dermal development to fine-tune endoplasmic reticulum function by regulating protein disulfide isomerase: B.H. Bin, et al.; J. Invest. Dermatol. 137, 1682 (2017), Application(s): ER fractions from murine osteoblasts, Abstract; |
11. | Succination of protein disulfide isomerase links mitochondrial stress and endoplasmic reticulum stress in the adipocyte during diabetes: A.M. Manuel, et al.; Antioxid. Redox Signal. 27, 1281 (2017), Application(s): 3T3-L1 adipocytes and adipose tissue lysates, Abstract; Full Text |
12. | Adverse outcomes associated with cigarette smoke radicals related to damage to protein disulfide isomerase: H. Kenche, et al.; J. Biol. Chem. 291, 4763 (2016), Abstract; |
13. | Adverse Outcomes Associated with Cigarette Smoke Radicals Related to Damage to Protein-disulfide Isomerase: H. Kenche, et al.; J. Biol. Chem. 291, 4763 (2016), Application(s): in-vitro assay with recombinant PDI, Abstract; Full Text |
14. | Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity: K. Mätlik, et al.; Cell Death Dis. 6, e2032 (2015), Abstract; Full Text |
15. | Methods of measuring protein disulfide isomerase activity: a critical overview: M.M. Watanabe, et al.; Front. Chem. 2, 73 (2014), Application(s): Review on available assays to measure PDI activity, Abstract; Full Text |
16. | N-octanoyl dopamine treatment of endothelial cells induces the unfolded protein response and results in hypometabolism and tolerance to hypothermia: E. Stamellou, et al.; PLoS One 9, e99298 (2014), Abstract; Full Text |
17. | The protein disulfide isomerases PDIA4 and PDIA6 mediate resistance to cisplatin-induced cell death in lung adenocarcinoma: G. Tufo, et al.; Cell Death Differ. 21, 685 (2014), Application(s): Evaluation of PDI activity in whole extracts and ER fractions, Abstract; |
18. | Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase: F. Langer, et al.; Blood 121, 2324 (2013), Abstract; |
19. | Oxidative processing of latent Fas in the endoplasmic reticulum controls the strength of apoptosis: V. Anathy, et al.; Mol. Cell. Biol. 32, 3464 (2012), Abstract; |
◆一般参考文献
1. | Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase: S. Abromaitis & R.S. Stephens; PLoS Pathog. 5, e1000357 (2009), Abstract; |
2. | Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages: C. X. Santos, et al.; J. Leukoc. Biol. 86, 989 (2009), Abstract; |
3. | The proteostasis boundary in misfolding diseases of membrane traffic: D.M. Hutt, et al.; FEBS Lett. 583, 2639 (2009), Abstract; |
4. | Increasing melanoma cell death using inhibitors of protein disulfide isomerases to abrogate survival responses to endoplasmic reticulum stress: P.E. Lovat, et al.; Cancer Res. 68, 5363 (2008), Abstract; |
5. | Targeting homeostatic mechanisms of endoplasmic reticulum stress to increase susceptibility of cancer cells to fenretinide-induced apoptosis: the role of stress proteins ERdj5 and ERp57: M. Corazzari, et al.; Br. J. Cancer 96, 1062 (2007), Abstract; |
6. | Coupling endoplasmic reticulum stress to the cell death program: R.V. Rao, et al.; Cell Death Differ. 11, 372 (2004), Abstract; |
7. | Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion: R. Barbouche, et al.; J. Biol. Chem. 278, 3131 (2003), Abstract; |
8. | Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells: C.H. Davis, et al.; Infect. Immun. 70, 3413 (2002), Abstract; |
9. | Protein-disulfide isomerase-mediated reduction of the A subunit of cholera toxin in a human intestinal cell line: P.A. Orlandi; J. Biol. Chem. 272, 4591 (1997), Abstract; |
10. | Acceleration of reactivation of reduced bovine pancreatic ribonuclease by a microsomal system from rat liver: R.F. Goldberger, et al.; J. Biol. Chem. 238, 628 (1963), Abstract; |
| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 备注 |
| ENZ-51024-KP002 | PROTEOSTAT PDI assay kit PROTEOSTAT PDI检测试剂盒 |
2×96 tests | ||
| ENZ-51024-KP050 | PROTEOSTAT PDI assay kit PROTEOSTAT PDI检测试剂盒 |
50 tests |
| 免责声明 |
|
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