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BAMBANKER® 无血清细胞冻存液
BAMBANKER®

BAMBANKER®新品速递图wako.jpg

无血清细胞冻存液

BAMBANKER® 是一种无血清细胞冻存液。可在-80℃长期保存细胞(肿瘤细胞和常规细胞)。

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◆产品特性


 ● 即用型细胞冻存液

 ● 无需分步降温,直接使用

 ● 无需稀释

 ● 无需程序降温盒

 ● -80℃长期保存

 ● 无血清




无血清冻存液的优点


 ● 与含血清类型相比,批次间的成分组成差异小,可保持稳定的品质。

 ● 不含血清,因此没有因动物源的未知成分和感染物质所产生的影响与风险。

 ● 可对无血清驯化细胞进行冷冻,节省再驯化步骤。

 



操作流程


1)收集生长对数期*的细胞(5×105-1×107个细胞)

2)用1mL该细胞冻存液悬浮细胞,置于冻存管中,不需预冷,直接-80℃冷冻保存,也可-80℃冻存12小时后可

      转移至液氮中保存。

3)用恒温箱或者水浴锅快速复苏细胞

      *冷冻细胞必须处于生长对数期


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无菌检测**


内毒素:生色底物法

支原体:荧光抗体法

真菌和细菌:依据日本药典

**:可索取检验证书)

 

 



BAMBANKER® Direct


BAMBANKER Direct是“无血清型”细胞冻存液。




BAMBANKER® Direct无需离心收集细胞


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① 不需冻存前预处理,操作简便

② 不需稀释,直接使用

③ 无需分步降温,直接使用

④ 可快速、长期冻存细胞(-80℃或液氮)

⑤ 不含血清

 


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BAMBANKER® Direct冻存步骤VS常规冻存步骤


使用本产品无需经过离心等复杂步骤,只需往培养基内添加与培养液等量的BAMBANKER Direct,再分装到冻存管,置于-80℃便可冻存细胞。

 



应用


BAMBANKER使用例】


 细胞名称 保存时间
生存率
  BAMBANKER 公司A(含血清)
公司A(不含血清)

P3U1

(小鼠骨髓瘤细胞系)

12个月
95%95%70%

K562

(人白血病细胞系)

12个月
73%70%60%
人体胃黏膜上皮细胞10个月
100%62%56%

human γδT cells

(人γδT细胞)

10个月
65%37%35%

 Daudi

(人 B细胞系)

12个月
100%100%92%

PC12

(大鼠源肾上腺嗜铬细胞瘤)

11个月
95%59%20%

human B cell line 

(人B细胞系)

9个月
74%54%35%

OKT4

(小鼠杂交瘤细胞)

12个月
100%100%92%

B细胞系

(猴)

10个月56%40%18%



低温冻存实验证明以下细胞保存完好

 

3T3‐L1(小鼠前脂肪细胞系)

A431(人扁平上皮癌细胞系)

BAEC(牛主动脉血管内皮细胞系)

Balb/3T3(小鼠成纤维细胞系)

C2C12(小鼠骨骼肌细胞系)

 Daudi(人B细胞系)

ECV304(人脐静脉内皮细胞系)

H295R(肾上腺皮质细胞)

HEK293(人胚胎肾细胞系)

HEK293T(人胚胎肾细胞系)

HeLa(人子宫颈癌细胞系)

HeLa S3(人子宫颈癌细胞系)

HepG2(人肝癌细胞系)

HFF(人正常成纤维细胞系)

Huh7(人肝癌细胞系)

Jurkat(人白血病T细胞系)

K562(人慢性骨髓性白血病细胞系)

KATOIII (人胃癌上皮细胞系)

KLM‐1(人胰腺癌细胞系)

MDCK(犬肾小管上皮细胞系)

MEF(小鼠胚胎成纤维细胞)

NIH3T3(小鼠胚胎皮肤细胞)

OKT4(小鼠杂交瘤细胞)

OP9(小鼠骨髓基质细胞)

P3U1(小鼠骨髓瘤细胞系)

PANC‐1(人胰腺癌细胞系)

PC12(大鼠源肾上腺嗜铬细胞瘤)

RPE(人视网膜上皮细胞系)

SNL(小鼠胚胎成纤维细胞)

TSU‐Pr1(人前列腺癌细胞系)

Vero(非洲绿猴肾细胞系)

human γδT cells (人γδT细胞)

human B cell line (人B细胞系)

HDF(人皮肤成纤维细胞) HCC20(人乳腺原发性导管癌细胞)BMMCs(人骨髓单核细胞系)
BMMCs(猪骨髓单核细胞系)BMSCs(马骨髓间充质干细胞系)C1(人成纤维细胞系)
CEF(牛胚胎成纤维细胞)CHO-K1(中国仓鼠卵巢细胞系)DPCs(大鼠牙髓细胞)
DPCs(人牙髓细胞)ESCs(人胚胎干细胞)EVT(人绒毛外滋养层细胞)
GH3(大鼠垂体瘤细胞)Gli36(胶质瘤细胞系)
h1(人类胚胎干细胞)
h9(人类胚胎干细胞)HN4(人口腔上皮细胞系)

HS-RMS-2

(多形性横纹肌肉瘤细胞系)

IPS(人诱导性多能干细胞)

LNCaP clone FGC

(人前列腺癌细胞)

MCF 10A(人正常乳腺细胞)

MEF-BL/6-1

(小鼠胚胎成纤维细胞)

MNCs(人单核细胞)MSCs(大鼠间充质干细胞系)
PBMCs(人外周血单个核细胞)PDL(人牙周膜细胞)pES(大鼠孤雌胚胎干细胞系)
Sf9(草地贪夜蛾细胞系)U251(胶质瘤细胞系)U87(胶质瘤细胞系)
VT(人绒毛膜滋养层细胞)癌症干细胞大鼠肝细胞

猴B细胞系

人外周血活化淋巴细胞

永生化人肌肉细胞

小鼠脾脏活化淋巴细胞

小鼠ES细胞系

人胃上皮细胞

大鼠神经祖细胞大鼠脂肪细胞狗肿瘤细胞
胶质瘤细胞系牛脂肪细胞牛子宫内膜上皮细胞
人扁桃体细胞人肝细胞人骨髓CD34+细胞
人巨噬细胞人淋巴细胞人输卵管上皮细胞
人胎儿卵巢成纤维细胞人胎儿卵巢体细胞人自然杀伤细胞
神经祖细胞小鼠颅骨成骨细胞心肌祖细胞
猪成纤维细胞

 

ES细胞(小鼠)使用实例
T.Hikichi,et al; Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 2007, 25, 46-53


更多相关资料请点击文字:

BAMBANKER® 与自制冻存液的冻存效果比较


Bambanker® 与其他相关产品的比较


细胞冻存效果验证


细胞冻存液类型

1.Bambanker®

2.Medium with serum (含血清,A公司)

3.Serum-free Medium (无血清,A公司)


实验结果

*1:细胞-80℃的保存时间


01.jpg



相关PDF


BAMBANKER单页 蓝色20190604-已压缩_页面_1.jpg


BAMBANKER® 无血清细胞冻存液

搜狗截图20170626173039.jpg


Wako BAMBANKER冻存液(新手册)

细胞种类列举

搜狗截图20171124122240.jpg


BAMBANKER细胞冻存液

-冻存解冻步骤说明

(终端).pdf






1.

Q:为什么我使用了BAMBANKER® 来保存细胞,但是存活率依然不高?

A:请冻存前确保细胞处于生长对数期,并且冻存时细胞数目控制在5×105~1×107/mL冻存  液。



2.

Q:我们实验室已经有固定的冻存程序了,换了你们的BAMBANKER® 可以还继续用原来程序降温的方法冻存吗?

A:虽然本产品可以无需程序降温冻存细胞,但如果通过程序降温盒等适当控制了温度下降的速度,效果更佳。



3.

Q:哪些细胞株(系)适合使用BAMBANKER® 来进行细胞冷冻保存?

A:几乎所有细胞株(系)都可以使用BAMBANKER® 进行冻存。对于较为宝贵的ES/iPS细胞的保存尤其适用。官网上所列举的细胞系均已经过测试验证。

但也并不排除可能有某些细胞株是不适合使用BAMBANER® 来进行冻存的,用户在没有确认是否可使用时,建议在进行正式细胞冻存之前先进行预实验。



4.

Q:无血清冻存液相比传统含血清冻存液有什么优势?

A:无血清冻存液因不含有动物血清,质量更稳定,批间差小;同时未知生物成分或感染性物质污染细胞的几率也极低,尤其对于ES/iPS细胞等有可能用于再生医疗的细胞安全得以严格保障;可以直接冻存无血清培养的细胞,免去无血清再驯化的步骤;另外BAMBANKER® 无血清细胞冻存液不需要像传统的血清冻存液需要程序降温,减少了用户的繁琐操作,节省了时间。



5.

Q:BAMBANKER® 在冷冻保存细胞的过程中起什么作用?

A:BAMBANKER® 无血清细胞冻存液使用了DMSO等作为保护剂,在冻存细胞时能以1℃/min左右的温度下降而逐渐冻结,在此过程中,细胞内的水分子被置换成冻结保护剂,抑制胞内和细胞周边的冰晶的形成,防止细胞膜和细胞器结构损伤,防止蛋白变质。



6.

Q:未使用的BAMBANKER® 无血清细胞冻存液应该如何保存?

A:2-10℃避光保存。开封后尽快使用。请注意保质期为自生产日期起24个月。



7.

Q:BAMBANKER® 能否使用于医疗领域?

A:BAMBANKER® 仅供科研使用,不能使用于人体或医疗领域。



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